Eukaryotic expression recombinant plasmids pcDNA3-ORF2,pcDNA3-V1,pcDNA3-V2,pcDNA3-V3 and pcDNA3-V1-V2 were constructed by using pcDNA3,PCV2 ORF2,and the variant regions of PCV2 ORF2 in order to develop DNA vaccines against porcine circovirus type 2(PCV2).

• 为研制猪2型圆环病毒(PCV2)新型核酸疫苗,以pcDNA3为载体,与PCV2的ORF2及其高变区V1、V2、V3、V1-V2连接,分别成功构建了真核表达质粒pcDNA3-ORF2、pcDNA3-V1、pcDNA3-V2、pcDNA3-V3和pcDNA3-V1-V2。