Embryonic callus of Kentucky bluegrass were used as material for genetic transformation with the DREB1A gene, BADH-CMO double-gene and CMO gene by particle bombardment. The transgenic plants were obtained.

 
  • 在此基础上,通过基因枪轰击法把与抗旱、耐盐有关的外源基因(DREB1A、BADH-CMO、CMO)对草地早熟禾胚性愈伤组织进行遗传转化,经过潮霉素筛选、PCR扩增与Southern杂交检测,获得了转基因植株。
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