Embryo implantation was examined at 16:00 and 20:00 of day 4, and at 8:00 of day 5 by intravenous injection of Chicago blue dye. The expression of both SNAIL and COX-2 at implantation sites was examined by immunohistochemistry.

• 在移植后第4天下午16:00和20:00,以及第5天上午8:00经尾静脉注射1%25芝加哥蓝后处死受体小鼠,通过剖检以确定胚胎着床情况,并对着床位点的切片进行组织学观察及免疫组织化学染色以研究SNAIL和COX-2的表达。