Egg yolk was diluted 10 times with distilled water, then frozen and thawed.WSF(water-soluble fraction) was separated through filtered through 0. 45um cellulose acetate membrane.

• 将高免卵黄液用水稀释10倍,冻融后经微滤分离出卵黄水溶性成分(WSF),分离WSF后的卵黄用溶剂法提取纯化卵磷脂。