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- For subcellular localization, TBX5 was expressed as an EGFP fusion protein in rat hepatocarcinoma cells.Results showed that TBX5 was nuclear. 构建荧光表达载体,转染大鼠肝癌细胞,观察TBX5亚细胞定位,结果证实TBX5基因在细胞核中表达;
- Objective: To express and purify fusion protein from angiogenin(ANG) and enhanced green fluorescent protein (EGFP) to further study the function of angiogenin. 目的:表达并纯化血管生成素(ANG)与增强型绿色荧光蛋白(EGFP)融合蛋白,以进一步探讨血管生成素的生物学功能。
- Purified fusion protein was obtained. 得到纯化的融合蛋白。
- EGFP alone failed to transduce into human aortic smooth muscle cells, whereas PEP-1-EGFP fusion protein could transduce into them and distributed in cytoplasm and nucleus after 1h incubation. EGFP蛋白不能进入细胞内,PEP-1-EGFP融合蛋白和人平滑肌细胞共同孵育1h后可见有明亮绿色荧光分布于胞浆和胞核。
- The GRA7 gene may be expressed as a GST fusion protein in E. coli. GRA7基因在大肠埃希菌中以GST融合蛋白的形式得到表达。
- The system uses the DirectX class for data acquisition form sound card, uses multi- thread for continuate acquisition,and gives a method of detecting DC sign. 使用DirectX技术从声卡采集数据,使用多线程技术实现数据的不间断采集,并给出了直流信号的数据采集方案。
- The fusion protein was purified by GSTrap FF affinity chromatography. GSTrap FF亲和层析柱纯化融合蛋白;
- The fusion protein was purified through Nickel-affinity chromatography column. 用镍柱纯化融合蛋白。
- Methods The recombinant fusion protein MBP hEra was expressed in the E. 方法在大肠杆菌中,表达重组MBP?hEra融合蛋白。
- Objective To construct the recombinant plasmid of eukaryotic expression vector containing M-CSF gene and transfect it into the NIH3T3 cells to study whether resulting in the expression of M-CSF and EGFP fusion proteins. 目的构建真核表达重组体pEGFP-M-CSF,并鉴定其在小鼠成纤维细胞NIH3T3细胞能否正确表达。
- BVP22 can mediate intercellular trafficking of fusion protein in vitro and in vivo. 在体内外BVP22均能够介导与其融合的蛋白在细胞间发生穿梭。
- The titer of antiserum was detected by double agar immunodiffusion with GST-PI fusion protein. 并用双向免疫扩散法鉴定家兔多价抗血清的效价。
- EGF-Ang fusion protein had definitely cytotoxicity to Hep2 cells which over expressed EGFR in vitro. 融合蛋白EGF-Ang在体外对过度表达EGFR的Hep2细胞具有明显的杀伤作用。
- Recombinant TrxA/PEB1 fusion protein could be a valuable candidate for CJ vaccine with great applied value. (4)PEB1融合蛋白作为CJ的亚单位侯选基因工程疫苗不仅可行,而且有良好应用前景。
- Results: The CAT gene was cloned correctly and it's fusion protein was expressed in E.coli. 结果:成功克隆了CAT的基因片段,并在大肠杆菌中得到其融合蛋白的表达。
- He persuaded her to sign the document by guile. 他用欺骗手段说服她在文件上签字。
- A novel fusion protein namely IL- 2-Ang consisting of interleukin-2 ( IL-2 ) and angiogenin ( Ang) was expressed in E. 利用基因工程技术在大肠杆菌表达成功人白细胞介素2(IL-2)和人血管生成素(Angiogenin)的融合蛋白(IL-2-Ang)。
- The s-lap/GFP fusion protein can express in Hela cells and locate in entire cells, but express highly in the nucleus. s- lap/ GFP融合蛋白可在人 Hela细胞中表达 ,并主要定位于细胞核。
- Western blot proved that the recombinant protein is the fusion protein of APE/Ref|1. Western印迹证明重组蛋白为APE ref 1融合蛋白 .
- Western-blot analysis with sera against CAV showed the fusion protein had good specificity. 在Western-blot免疫印迹分析中,3个融合蛋白与CAV阳性血清均能发生特异性反应,表明它们都具有良好的反应性。