EGFP was expressed both in X4550 and COS 7 cell and its expression level was comparable with corresponding recombinant S.typhimurium X4550 (pYA3334 EGFP) and EGFP eukaryotic expression plasmid pVAX1 EGFP.

• 将质粒p VAXD-EGFP转染 COS-7细胞 ,EGFP可在 COS-7细胞核和胞浆表达 ,在荧光显微镜下发出强烈荧光。