E), alkali phosphatase (ALP) was assayed by biochemistry analysator, at last the number of CD4+ T, CD8+ T cells and the CD40L expression in T cells were analyzed by flow cytometry (FCM).

• E染色观察胆管病理变化，生化分析仪测定血清碱性磷酸酶(ALP)含量，流式细胞术分析外周血CD4+、CD8+T淋巴细胞所占比例及CD40L在T细胞表面的的表达情况。