During the artifical antigen preparation, room temperature oximation reaction monitored by thin layer chromatography (TLC) assay was used to get AFB_1-Oximation (AFB_1-O) instead of heating flux oximation reaction, the yield was up to 95%.

• 在AFB_1人工抗原制备的第一步肟化反应中,用室温肟化法代替传统的加热回流法,使肟化率达到95%25;