During early embryo development, Foxd3 mRNA has been detected in blastocyst-stage embryos and throughout the epiblast of the 6.5 days postcoitum (dpc) embryo.

• 实验发现Foxd3基因敲除的胚胎在E6.；5天死亡；并且表现为epiblast的减少；取而代之的是近端胚外组织(proximal extraembryonic tissue)。