Double PCR was carried out by using both PLxx and P4 .Dot-blot of Lxx was set up by the probe prepared from cloned plasmid.

• 以含PLxx扩增产物的重组质粒为模板，随机引物法制备地高辛核苷酸标记探针，建立了Lxx的核酸斑点杂交检测技术。