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- Several differentially expressed genes according to the microarray results were confirmed by real-time PCR and IHC. 荧光定量PCR及免疫组织化学结果和芯片结果一致。
- Fluorescent real-time quantitative PCR was used to investigate some of the differentially expressed genes. 对部分筛选出来的差异表达基因使用荧光定量PCR方法检测并确认其变化;
- The Student’s t statistic is used in one stage log-ratio models to identify differentially expressed genes. 混合机率分布模型则是将基因表现分成表现有显著差异及没有显著差异两个族群来作讨论。
- Objective This study aims to screen the differentially expressed genes of glioblastoma using microarray technology. 目的用生物芯片筛选胶质母细胞瘤相关基因。
- Conclusion Many differentially expressed genes related to signal transduction play a role in the development of bladder transitional cell carcinoma. 结果膀胱肿瘤的发生与发展与多种细胞信号转导相关基因的异常表达有关。
- Objective To identify differentially expressed genes between chronic hepatitis B(CHB) patients and asymptomatic HBV carriers(ASCs). 目的筛选慢性乙型肝炎(chron ic hepatitis B,CHB)患者与无症状乙型肝炎病毒携带者(asymptom atic HBVcarriers,ASCs)表达差异的基因。
- The resulting products, now enriched for differentially expressed genes, were PCR amplified using primers that matched the sequence of the adapters. 将PCR产物用Qiagen胶提试剂盒纯化,连接到PT7Blue T vector(Novagen),做序列分析,并用GCG软件在基因库中比较;
- Objective To study the differentially expressed genes in the vascular tissue of diabetic gangrene toot and the possible mechanism of diabetic gangrene. 摘要目的研究严重糖尿病足坏疽血管组织中的差异表达基因及糖尿病坏疽的可能机制。
- Three of the 4 differentially expressed genes in rice were highly expressed in stamen and the remaining one in young panicle. 拟南芥4个差异基因都在花粉管高表达, 水稻4个差异基因有3个在雄性花蕊中高表达, 另一个在幼穗中高表达。
- Objective To screen the differentially expressed genes between esophageal carcer and carcer adjacent tissue using cDNA microarray. 目的应用微矩阵表达谱基因芯片研究食管癌的基因变化。
- BACKGROUND & OBJECTIVE: Subtractive hybridization technology is a common method to screen and clone differentially expressed genes. 背景与目的:消减杂交技术是一种寻找和克隆差异基因的方法。
- Ding. 2008.Association of single nucleotide polymorphism in the embryonic differentially expressed genes with reproductive traits in pigs. 台湾土鸡表现序列标签序列库之单一核苷酸多型性的侦测。
- In the first step, significant analysis of microarrays (SAM) was used to select a subset of differentially expressed genes (DEGs). 第一步,采用基因表达差异显著性分析方法(SAM)筛选得到差异表达基因子集。
- Of 384 screened genes, 33 differentially expressed genes related to EOC cell line were found, and 22 genes were up-regulated and 11 were down-regulated. 在384条侯选基因中,与卵巢癌相关的差异表达基因33条,其中22条表达上调,11条表达下调。
- Suppression subtractive hybridization (SSH), which is feasible and highly specific, is one of the most efficient methods for cloning differentially expressed genes. 摘要抑制性消减杂交是一种简便易行、特异性高的高效分离差异表达基因的方法。
- In this study, we first applied cDNA microarray technique to analyze the differentially expressed genes between 2 pairs of AAA and normal aortic tissues. 本研究首先应用基因芯片技术对2对AAA和正常主动脉组织进行了基因差异表达分析。
- Methods The differentially expressed genes in the vascular tissue of diabetic gangrene foots were screened by the functional classific gene chip. 方法应用功能分类基因芯片筛选糖尿病坏疽血管组织中的差异表达基因;
- To identify differentially expressed genes is an important step to better understand the molecular mechanisms in the tumorigenesis and tumor progression of HCC. 为了更进一步瞭解肝细胞癌肿瘤发生与肿瘤进行过程的分子机制,分析并且辨别差异性表现的基因是非常重要的步骤。
- Suppression subtractive hybridization(SSH),which is feasible and highly specific,is one of the most efficient methods for cloning differentially expressed genes. 抑制性消减杂交是一种简便易行、特异性高的高效分离差异表达基因的方法。
- RESULTS: Of 384 screened genes, 33 differentially expressed genes related to EOC cell line were found, and 22 genes were up-regulated and 11 were down-regulated. 结果:在384条侯选基因中,与卵巢癌相关的差异表达基因33条,其中22条表达上调,11条表达下调。