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- Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid. 方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法。
- In GENETIC ENGINEERING, the joining of DNA fragments by the action of the enzyme DNA ligase. 在基因工程中，在DNA连接酶的作用下连接DNA片段的过程。
- With T4 DNA ligase link the GFP gene to pET-MP, the constructed plasmid named after pET-MP-GFP. 用T4连接酶将gfp基因连接到pET-MP上，得到的质粒命名为pET-MP-GFP。
- Once a plasmid is snipped open, a foreign piece of DNA, cut by the same enzyme scissors, can be taped, end to end, into the plasmid using another enzyme, DNA ligase. 一旦原生质被分开后，用同样限制酶剪开的外来DNA片段和它的被切断的端口被粘到一起，形成了新的原生质。连接时使用的是另一种酶--DNA连接酶。
- DNA ligase An enzyme, used in DNA REPLICATION and the in vitro synthesis of DNA, that joins together segments of newly synthesized polynucleotide chains using energy from ATP. DNA连接酶：是一种在DNA复制或DNA晶体合成中起作用的酶，它能够将新合成的DNA片段连接在一起，反应中需要ATP提供能量。
- DNA ligase An enzyme, used in DNA REPLICATION and the in vitro synthesis of DNA, that joins together segments of newly synthesized polynucleotide chains using energy from ATP. See replication. DNA连接酶：是一种在DNA复制或DNA晶体合成中起作用的酶，它能够将新合成的DNA片段连接在一起，反应中需要ATP提供能量。参见replication.
- Recent Advances in DNA Ligase Isozymes DNA连接酶同工酶的研究进展
- Keywords molecular beacon;DNA ligase;polymerase;biomolecule;detection; 分子信标;连接酶;聚合酶;生物分子;检测;
- Keywords Plasma DNA;Point mutation;DNA ligase;Real-time PCR;Capillary electrophoresis;Gene array; 血浆DNA;点突变;DNA连接酶;荧光定量PCR;毛细管电泳;基因芯片;
- Comparison between the T_4DNA ligase activity determined by DNA ligase self-adenylation and ATP-~(32)PPi exchange reaction T_4DNA连接酶活性的自身腺苷化和交换法测定的比较
- Biochemical and Enzymatic Characterization of a Thermostable DNA Ligase Encoded by Thermophilic Acidophilic Archaebacterium Strain JP2 一种嗜热耐酸古细菌JP2菌株编码的热稳定DNA连接酶的生物化学及酶学特性研究(英文)
- Keywords DNA Ligase;Alkaline phosphatase;biocatalytic deposition;point mutation;genotype;electrochemical immunoassay;catalytic hydrogen electrochemical determination; 关键词DNA连接酶;碱性磷酸酶;生物催化沉积;点突变;基因分型;电化学免疫试验;催化氢波电化学检测;
- El triplete de Liga, Copa y Champions sigue a tiro. 巴萨进入了欧冠决赛，继续着他们三冠王的梦想。
- Primera Liga Zhanmu open, beautiful Mata continue. 与一战，马塔83分钟的绝杀为瓦伦西亚拿回3分。
- Liga_5_Star Spletna igra Liga 5 Star. 东莞豪华大酒店四星级园林式酒店。
- Keywords Single Nucleotide Polymorphisms （SNPs）;DNA ligase;Colorimetric;Molecular beacon;Aptamer beacon;Bio-species immobilization;Biosensor;Immunoassay; 单核苷多态性;DNA连接酶;比色法;分子信标;分子信标识体;生物组分固定化;生物传感器;免疫分析;
- DNA fingerprinting can help the police to find the criminals. DNA指纹术有助於警察发现罪犯。
- The mutant DNA miscoded a single amino acid. 突变体脱氧核糖核酸误编了一个氨基酸密码。
- Spanish Primera Liga player Andres, D'Alessandro and former U. 由于传递线路周围人流涌动，遍地都是欢迎圣火的人群，导致交通阻塞，火炬接力进展缓慢。
- dna ligase dna 连接酶
