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- Method: PCR amplification,DNA sequencing and cladistic analysis. 方法:PCR扩增、DNA序列测定、分支分析。
- DNA sequencing DNA 序列测定
- HBeBP4A gene was successfully amplified and identified by DNA sequencing. 成功扩增出HBeBP4A基因,测序结果符合GenBank报告序列。
- Laboratory diagnosis of Hgb Hekinan using HPLC and DNA sequencing methods? 施浤彰(Hung-Chang Shih);施木青(Mu-Chin Shih);张煜昌(Yu-Chang Chang);林惠茹(Hui-Ju Lin);林彩秀(Tsai-Hsiu Lin);彭庆添(Ching-Tien Peng);张天杰(Tien-Jye Chang);张建国(Jan-Gowth Chang)*?
- And climbing it requires the development of a lot more technological hicks than did sequencing DNA. 爬这座山需要发展出比测序DNA多得多的技术手段。
- Sequencing DNA has come down in price by half annually, from $10 per base pair in 1990 to under a penny today. 资讯科技通常不要一年,价格表现比和产能就会增加一倍。
- The methylation specific PCR (MSP) and bisulfite DNA sequencing were performed to examine the methylation status of SLIT2 gene promoter. 亚硫酸氢钠处理DNA,用MSP和测序法检测SLIT2启动子甲基化状态。
- New primers were designed for hot - start PCR reaction according to the results of DNA sequencing. 根据测序结果设计新的引物以进行热启动PCR反应;
- It was proved that the E2 genes were integrated stably into chromosome of P.Pastoris by Dot blot and DNA sequencing. Pastotis进行整合,经G418筛选得到25个高拷贝转化子,经DNA斑点试验和DNA测序证明外源基因E2稳定地整合到P.;Pastoris染色体中。
- A deletion mutation with frameshift of neurofibromatosis type 2 gene was detected by DNA sequencing. 以DNA序列分析出,在第二型纤维细胞瘤基因上发生染色体缺失造成移码突变。
- Authentication,PCR and DNA sequencing showed the recombinant plasmid of human MIA/CD-RAP was successfully constructed. 酶切电泳和DNA测序结果表明,成功地克隆了人黑素瘤M IA/CD-RAP cDNA。
- B7 1(CD80) cDNA was cloned by RT PCR from human B lymphma Raji cell line and confirmed by DNA sequencing. 应用RT-PCR技术从人B淋巴瘤细胞系Raji中克隆到B7-1(CD80)cDNA,并经测序证实。
- Methods STK 11 germline mutation was analysed by DNA sequencing in 18 unrelation patients with PJS. 方法 用 DNA直接测序方法 ,对 18个家系的 PJ综合征患者 STK1 1 基因 9个外显子进行研究。
- Detection of keratin 5 gene point mutation in a family with Weber Cockayne EBS by PCR and direct DNA sequencing. PCR-DNA直接测序检测1例单纯型大疱性表皮松解症Weber-Cockayne亚型(WC-EBS)患者角蛋白K5基因点突变
- The more varied the organisms, the more varied the DNA sequences. 生物变化越多,DNA序列的变化也就越多。
- Methods:PCR,denatur ing gradient gel electrophoresis (DGGE) atd DNA sequencing were used. 方法:采用多聚酶链反应(PCR),变性梯度凝胶电泳(DGGE)和DNA测序等方法对上海地区血友病甲基因突变进行检测。
- The more varied the organisms,the more varied the DNA sequences. 生物变化越多,DNA序列的变化也就越多。
- DNA microarray has been applied to DNA sequencing,pharmaceuticals analysis,gene expression and so on. DNA微集阵列已广泛用于DNA测序、药物分析、基因表达等研究领域。
- In contrast, direct DNA sequencing requires only a single sample from the proband. 相比之下,直接测序只需要被检测者的血样 。
- One relied on the DNA sequence of a gene called cytochrome oxidase. 第一种方式是根据一种叫做细胞色素氧化酶基因的DNA序列。