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- After transfection of pPTA1/Ig into COS 7 cells by DEAE dextran, expression of a secreting fusion protein was identified by affinity chromatography and sandwich ELISA assay with anti PTA1 mAb and HRP anti hIgFc mAb. 重组载体通过DEAE?dextran法转染COS?7细胞,经夹心ELISA及亲和层析、SDS?PAGE鉴定融合蛋白的表达及免疫学活性。
- DEAE dextran 二乙氨(基)乙基葡聚糖
- Pinocytosis of dextran was unaffected. 对葡聚糖的胞饮作用无影响。
- After purification with DEAE Sepharose F. 结果:成功得到海螵蛸粗多糖。
- Dextran sulfate sodium salt from Leuconostoc spp. 硫酸葡聚糖。
- Ingredient:Plant coenzyme Q10, Extended protein factor, dextran. 成份:植物辅酶Q10,伸展蛋白因子,葡聚糖。
- Methods: The polysaccharide was got by the extraction, DEAE chromatography etc. 方法:依次用水提取、过滤、去蛋白、DEAE纤维素层析、冷冻干燥获得大蒜多糖;
- Methods Clinical study was made in 13 cases with ARF by low Molecular Dextran. 方法:回顾性分析13例低分子右旋糖酐致急性肾功能衰竭的临床资料。
- Iron Dextran manufactured by Herbon is the best iron product in the world. 汉邦公司生产的右旋糖酐铁是世界上最好的用于防治动物及人体贫血的药物之一。
- The concentration of dextran solution was measured by testing its turbidity value using ethanol as the precipitant. 以乙醇为沉淀剂;测定水溶液中葡聚糖溶液浊度的方法测定葡聚糖的浓度.
- For DEAE Sepharose FF weak anion-exchange resin chromatography, the optimum operation was with buffer Tris-HCl(Ccl_ =20mM),pH8.0 and the concentration of NaCl was also 0.4M. 对于DEAE弱阴离子交换层析柱,采用Tris-HCl(Ccl_ =20mM),pH8.;0缓冲体系;同时洗脱缓冲体系也维持NaCl浓度为0
- Comparison of homogeneous HDL-C assay with dextran sulfate 50- magnesium chlorideprecipitation method. 高密度脂蛋白胆固醇直接测定法与硫酸葡聚糖50-镁法比较。
- RESULTS:ADM attached to 5F11 with a glutaraldehyde bridge (5Fll-ga-ADM),and a dextran bridge (5Fll-dex-ADM). 集落形成试验结果为:对于801-D细胞,ADM及ADM+5F11的100%25杀伤浓度均为1ug/ml,而5F11-ADM则为0.;1 ug/ml;降低了10倍(P<0
- Method The contents of Fe and dextran were determined respectively by o-phenanthroline and anthrone methods. 方法采用邻二氮菲法及蒽酮法分别测定铁及右旋糖酐含量。
- Methods The neuronal tract tracing by applying HRP(horseradish peroxidase),BDA(biotinylated dextran amine). 方法 HRP和生物素结合的葡聚糖胺(BDA)的神经示踪技术。
- Methods:A neural tracer biotionylated dextran amine (BDA) method,and immunohistochemical method were used. 方法 :采用生物素结合的葡聚糖胺 (BDA)双向示踪方法和P物质 (substanceP ,SP)免疫组织化学技术。
- Objective To determine the application of modified low-potassium dextran (LPD) solution in heart-lung transplantation. 目的:探讨改良低钾右旋糖酐(LPD)溶液在心肺移植术中的肺保护作用。
- The method was compared with ABC method and dextran coated charcoal bio-chemical assay (DCC). 以抗雌二醇ABC免疫组化法(ABC法)和葡聚糖包裹活性炭生化法(DCC法)作为E_2-HRP法的对照。
- METHODS Dexamethasone succinate dextran was synthesized in an anhydrous environment. 方法通过丁二酸酐搭桥,合成地塞米松葡聚糖酯前体药物;
- Cerebral microcirculatory disorder was made by intravenous injection of high molecular weight dextran (HMWD). 结果两药均可增加正常状态下和微循环障碍时小鼠脑微区血流量。
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