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- After transfection of pPTA1/Ig into COS 7 cells by DEAE dextran, expression of a secreting fusion protein was identified by affinity chromatography and sandwich ELISA assay with anti PTA1 mAb and HRP anti hIgFc mAb. 重组载体通过DEAE?dextran法转染COS?7细胞，经夹心ELISA及亲和层析、SDS?PAGE鉴定融合蛋白的表达及免疫学活性。
- DEAE dextran 二乙氨(基)乙基葡聚糖
- Pinocytosis of dextran was unaffected. 对葡聚糖的胞饮作用无影响。
- After purification with DEAE Sepharose F. 结果：成功得到海螵蛸粗多糖。
- Dextran sulfate sodium salt from Leuconostoc spp. 硫酸葡聚糖。
- After ATF gene transfection, the ATF mRNA was obviously expressed. 结果表明,转染ATF基因后,肿瘤细胞呈现ATF基因的明显表达;
- Transfection, make stable C/EBP p expression cell line. 质粒转染，制备稳定表达C尼BPp的细胞系。
- Cell density was associated with PEI/DNA transfection efficiency. 细胞密度影响转染效率;
- Gene transfection is the key technique of gene therapy. 摘要基因治疗的关键技术是基因转移。
- Ingredient：Plant coenzyme Q10, Extended protein factor, dextran. 成份：植物辅酶Q10，伸展蛋白因子，葡聚糖。
- Methods: The polysaccharide was got by the extraction, DEAE chromatography etc. 方法:依次用水提取、过滤、去蛋白、DEAE纤维素层析、冷冻干燥获得大蒜多糖;
- Objective To construct Giardia canis virus(GCV) transfection vector. 目的构建犬贾第虫病毒(Giardia canis virus,GCV)转染载体。
- Methods Clinical study was made in 13 cases with ARF by low Molecular Dextran. 方法:回顾性分析13例低分子右旋糖酐致急性肾功能衰竭的临床资料。
- Iron Dextran manufactured by Herbon is the best iron product in the world. 汉邦公司生产的右旋糖酐铁是世界上最好的用于防治动物及人体贫血的药物之一。
- GFP can be used as a reporter system for optimizing transfection of K562 cells. 结论：电转染较脂质体转染K562细胞的效率高，GFP可作为报告系统优化K562细胞转染条件。
- The inhibitory effect of gene transfection on solid tumor was observed in mice. 小鼠实体瘤块模型研究基因转染抑制肝癌生长作用。
- Hoechst and HE staining showed that apoptosis was induced after transfection. 细胞HE染色部分细胞呈典型的凋亡细胞形态学改变；
- The concentration of dextran solution was measured by testing its turbidity value using ethanol as the precipitant. 以乙醇为沉淀剂;测定水溶液中葡聚糖溶液浊度的方法测定葡聚糖的浓度.
- For DEAE Sepharose FF weak anion-exchange resin chromatography, the optimum operation was with buffer Tris-HCl(Ccl_ =20mM)，pH8.0 and the concentration of NaCl was also 0.4M. 对于DEAE弱阴离子交换层析柱，采用Tris-HCl(Ccl_ =20mM)，pH8.;0缓冲体系;同时洗脱缓冲体系也维持NaCl浓度为0
- Comparison of homogeneous HDL-C assay with dextran sulfate 50- magnesium chlorideprecipitation method. 高密度脂蛋白胆固醇直接测定法与硫酸葡聚糖50-镁法比较。
