DDA was attached to Bovine serum albumin(BSA) and Ovalbumin(OVA) using N,N-dicyclohexylcarbodiimide,during which the synthesis of hapten DDA-BSA and DDA-OVA was gained.

• 采用碳二亚胺法,将半抗原DDA分别与载体蛋白牛血清白蛋白(Bovine serum album in,BSA)和卵清蛋白(Ovalbu-m in,OVA)偶联,得到包被抗原和免疫抗原。