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- CsCl Prep of Plasmid DNA 质粒提取
- Objective To develop a procedure for large-scale purification of plasmid DNA. 摘要目的建立适合大规模纯化质粒DNA的工艺。
- This arti cle reviews the structure of plasmid DNA, especially the structure of its Assist ant units and effects. 本文阐述了基因免疫中质粒DNA中的结构组成特别是其佐剂单位及其作用。
- Routine alkaline lysis miniprep of plasmid DNA was modified,and LiCl was specially used. 对碱裂质粒小量法进行了改进，并且在提取过程中增加了LiCl处理。
- TNA may enhance transfection rate of plasmid DNA mediated with liposome, and may be beneficial to the treatment of cancer. TNA可促进脂质体介导质粒DNA转染结直肠癌细胞 ,将营养支持与基因治疗相结合 ,有望提高对肿瘤患者支持治疗效果。
- A variety of approaches have been taken to augment and modulate the immunogenicity and efficacy of plasmid DNA vaccines. 核酸疫苗虽然在小动物实验中取得成功,但对大动物、灵长类动物和人类的免疫原性却很低。
- The damage effect of plasmid DNA and mutation induced of lacZ gene were analysesed by low power HeNe laser radiation invitro plasmid DNA. 利用低功率HeNe激光辐照离体质粒DNA，分析了质粒DNA的损伤效应和lacZ基因的突变。
- Methods Agarose gel electrophoresis of plasmid DNA, adherence test, and extract of plasmid were used.Electron microscope photos were prepared and observed. 方法：应用琼脂糖凝胶电泳试验、电镜照片制备及观察、粘附试验及质粒消除试验。
- The ability of amphiphilic block copolymers that consist of polyethylenimine (PEI) and poly(L-lactide) (PLLA) to modulate the delivery of plasmid DNA was evaluated. 本文对由PEI和PLLA组成的两性分子嵌段共聚物调节质粒DNA转运的能力进行了评估。
- Plasmid DNA has important function of gene regulation, direct injection of naked or Lipid - coated plasmid DNA often requires considerable amount of plasmid DNA. 质粒DNA有重要的基因调节性能，直接注射裸露或用脂质体包埋的质粒DNA常需要大量的质粒DNA。
- The size of these key components means that their susceptibility to fluid mechanical forces during processing is probably the most critical issue in the large-scale preparation of plasmid DNA. 这些关键构质得大小意味着：在DNA质体大规模准备活动中，这些关键构质在形成过程中对于流性机械力的易感性将起决定因素。
- Concerning herpesvirus infections, several reports have mow described the use of plasmid DNAs encoding herpesvirus proteins to induce a protective immunity in mice. gB蛋白诱导免疫保护作用具有长时效特点，明显保护三叉神经节免于感染； 也有实验表明鼠接种分泌形式的gB蛋白对由HSV-1感染引起的角膜炎，脑膜炎和死亡具有明显保护作用；
- PREPARATION OF PLASMID DNA PROBE OF PLASMODIUM FALCIPARUM 恶性疟原虫特异的质粒型DNA探针的制备
- DNA Rapid Preparation of Plasmid DNA for Overnight Restriction Digestion 快捷提取能经受过夜酶切的质粒
- In the 6th week plasmid DNA could only be detected in quadriceps. 第6周除在股四头肌可检测到外,其余组织中均为阴性;
- Methods tor Rapid Identification and Purification of Plasmid DNA in Large Quantity 快速鉴定和大量纯化质粒DNA的方法
- A Rapid Extraction of Plasmid DNA and Its Application in Mass Screening of Recons 质粒DNA的快速提取及其在重组子大量筛选中的应用
- DNA Large-scale Purification of Plasmid DNA by Ultrafiltration with Hollow Fiber Filter and Hydrophobic Chromatography 中空纤维超滤和疏水层析大规模纯化质粒
- DNA Reconstruction of plasmid DNA with Red-mediated homologous recombination combined with restriction digestion 用Red介导的同源重组结合酶切改构质粒
- Evaluating the Harmfulness of Copper in Aquatic Environment by Measuring the Supercoiled Structure Change of Plasmid DNA 水环境中铜离子有害性的DNA表征方法
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