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- Staining: with silver and coomassie brilliant blue staining. 染色:行银染和考马斯亮蓝染色;
- Un-adsorbed protein was detected by coomassie brilliant blue G-250 method. 用考马斯亮蓝G-250法检测未反应的蛋白质。
- The binding reaction of the dye coomassie brilliant blue with proteins have been studied. 研究了考马斯亮蓝与蛋白质的结合反应。
- The results were compared with traditional method(Coomassie Brilliant Blue R250). 并和考马斯亮蓝R250染色方法比较。
- Coomassie Brilliant Blue method was adopted to detect the protein content of seventeen kinds of allergens. 采用考马斯亮蓝G250检测17种变应原的蛋白质含量,与重量/体积法对照,发现按后者配制的变应原皮试液蛋白质含量悬殊。
- It involves the binding of Coomassie Brilliant blue to protein。However, detergents such as sodiu... 摘要:The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration。
- Protein crystals, large or small, can be stained with Mercury bromphenol blue, Amido black 10B and Coomassie brilliant blue R250 or G250 but salt crystals are not. 本文报导了蛋白质晶体能被汞溴酚蓝、氨基黑10B和考马斯亮蓝R250或G250染色;而无机盐晶体不被染色.
- The purified GAD showed two main bands,67 kD and 44 kD,on SDS-PAGE by Coomassie Brilliant Blue R-250 and Western-Blot under reducing condition. 纯化的GAD在变性条件下电泳,经考马斯亮蓝R2 5 0染色以及Western Blot鉴定主要有两条带,分子量分别为6 7kD和4 4kD。
- In addition, the staining of mPEG with BaCla and la is more specific and sensitive than the Coomassie Brilliant Blue staining. SDS-PAGE采用BaCl_2和I_2对mPEG染色比考马斯亮蓝染色水蛭素更为灵敏,特异性也很高。
- The expressed protein yield reached 385mg/L in the 10L fermentor assayed by Coomassie brilliant blue stain G-250 method. 该条件用于指导小试(5L/10L罐),蛋白表达量达到了385mg/L;
- Then the gels were stained by Coomassie brilliant blue R-250,and analyzed using Image Master 2D Platinum v 5.0 software. 运用Image Master 2D Platinum v 5.;0凝胶图像分析软件对2-DE凝胶图像进行差异表达分析
- Method The Coomassie Brilliant Blue G250 was dissolved into the ethanol and phosphate,then the SDS-PAGE was stained with the solution. 方法用考马斯亮蓝G250和磷酸作为染色液,用水作为脱色液。
- Appearence, location and arrays of cortical microtubules are consistent with those of cellular network visualized by staining of Coomassie brilliant blue R250. 其出现时间、布状况以及排列形态,基本上与考马斯亮蓝染色法观察到的网状结构物相一致。
- The results of determination for three serum samples were identical with those obtained according to the Bradford method using Coomassie Brilliant Blue (CBB G-250). 本方法成功应用于三个血清样品中蛋白质总量的测定,其结果与考马斯亮蓝法(Coomassie Brilliant Blue,CBB G-250)测定结果一致。
- BSA was run on SDS- PAGE, stained with Coomassie Brilliant Blue(CBB) G- 250, or reverse stained with Imidazole- Zinc, then recovered by using electroeluter. 以牛血清白蛋白(BSA)为材料进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE),分别采用考马斯亮蓝(CBB)G-250染色和咪唑-Zn反染,用电洗脱仪回收。
- Methods:The BSA standard protein was incised from 2-DE gel by Coomassie Brilliant Blue dyeing and was mixed with matrix (CHCA),then analyzed by MALDI-TOF MS. 方法:从考马斯亮蓝染色的2-DE胶上取下牛血清白蛋白(BSA)标准蛋白进行胶内原位酶切,与基质(CHCA)混匀后进行MALDI-TOF MS分析;
- All stylet-secretions can be stained with coomassie brilliant blue G-250 which specially dye protein, therefore, stylet-secretions must contain proteins. 此外,5个株系均对考马斯亮蓝敏感,而考马斯亮蓝是特异染蛋白的染料,因此,可证明口针分泌物中存在蛋白质。
- Methods Semen smears from 55 male infertility patients were stained according to Wright-Giemisa, Modified Papanicolaou and Coomassie brilliant blue methods. 方法:分别采用改良巴氏染色法、瑞-吉染色法和考马斯亮蓝染色法对55例男性不育症患者精液涂片染色,计算形态正常精子率和顶体完整率。
- The structural characteristics of the microfilaments were revealed in sterile pollenes of CMS, CHA and mature fertile on wheat by Coomassie brilliant blue R250. 以T型细胞质雄性不育(CMS)、化学杂交剂(CHA)诱导的雄性不育及成熟可育的花粉粒为材料,用考马斯亮蓝R250显示细胞内微丝的方法,观察分析了3种类型花粉粒内微丝结构的变化。
- In this article a modified method of in-gel digestion of protein spots excised from Coomassie brilliant blue stained two-dimensional(2-D) electrophoresis gels is introduced. 介绍一种简便高效的从考马斯亮蓝染色的双向电泳凝胶切取蛋白质点,通过胶内酶切制备基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)样品的方法。