Consequently, the cloning and expression of the gene encoding the amidase was focused and emphasized in this thesis.

• 为了阻断丙烯酰胺生产中的副产物生成或强化丙烯酸生产中的产物合成，本研究重点对诺卡氏菌酰胺酶基因的分子克隆和重组表达进行了研究。