Conclusion: The eukaryotic expressed vector pIRES2-EGFP-5HRE-AFPp-NTR was successfully constructed, which makes it possible to futher investigate its role in vitro.

• 结论：成功构建了真核表达载体pIRES2-EGFP-5HRE-AFPp-NTR，为下一步的体外及体内实验奠定基础。