Conclusion The bacterial RNA detected by PCR or RT-PCR in middle ear effusion seems not from live bacteria, but from bacterial scraps before the development of effusion.

• 结论应用PCR和RT-PCR技术在大多数中耳分泌液中检测到的细菌RNA不是来自于活的细菌，可能是受到中耳分泌液保护的以前细菌感染的残余物。