Collage n synthesis and expression of collagen IV and FN mRNA were investigated by 3 H-proline incorperation an d RT-PCR .

• 采用3H－脯氨酸参入法测定总胶原合成，ELISA方法测定细胞培养上清中IV型胶原和板层素浓度，RT－PCR技术观察HKC细胞中IV型胶原及纤维连接蛋白(FN) mRNA表达。