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- The co expression plasmid encoding FasL and VEGF165 named pCI FasL IRES VEGF165 (simplified as pCI FIV) was successfully constructed. 再以此为基础构建得到编码FasL和VEGF165的共表达质粒 pCI FasL IRES VEGF165 (简称pCI FIV )。
- Results The bicistronic eukaryotic expression plasmid was corrected and can co - express human BMP -2 and VEGF165 mRNA in vitro. 该重组质粒能在体外同时表达BMP2及VEGF165 mRNA。
- In addition, a set of expression plasmid vectors, PMS-31b. 同时构建一组质粒表达载体PMS-31b。
- Constructed the expression plasmid pTrc-rCR and checked it. 构建表达质粒pTrc-rCR,并酶切检验;
- The co expression rate was 71%, which was positively related with tumor grade. Fas、FasL共同阳性率 (共表达率 )为 71%25 ,与肿瘤级别呈正相关。
- Objective To clone the human angiotensin-converting enzyme 2 (ACE2)and construct its eukaryotic expression plasmid. 目的克隆人血管紧张素转换酶2基因(ACE2),并构建其真核表达载体。
- Results: Recombinant expression plasmid of ALR was confirmed correct by restriction enzyme digestion and sequencing. 结果：酶切鉴定及测序结果提示表达产物正确；
- CD gene can not only integrate and co express in mice bone marrow cells,but also increase the drug resistance to Ara C. CD基因可以进入小鼠骨髓细胞并且获得共表达,提高了造血细胞对阿糖胞苷的耐药性。
- Results showed that IRES was superior to SV40 promoter for guarantee of genes co expression in bicistronic retrovirus mediated gene therapy. 结果表明:在双基因逆转录病毒载体介导的小鼠骨髓细胞的基因转导中,IRES与内部SV40启动子相比,更能保证双基因的共同表达。
- Expression and purification of recombinant RTAsThe expression plasmid Pkk223.3-RTA was introduced into E. coli JM 109 by CaCl2-mediated method. 将构建好的重组质粒pKK223.;3-RTA和pKK223
- In certain neurons of paraventricular and supraoptic nuclei, co expression of Fos protein and vasopressin was induced by SFO stimulation. 免疫组化双重染色结果显示 ,刺激SFO能诱导视上核和室旁核中部分神经元呈Fos蛋白和加压素共同表达。
- The experiment results covered several following points:(1) adw was cloned into nuclear plasmid PB, PBG and chloroplast expression plasmid PLCTB . 将乙肝表面抗原基因(adw型)与细胞核载体PB、PBG以及叶绿体表达载体PLCTB进行定向克隆，经PCR和测序鉴定都获得了重组子，分别命名为PB-adw、PBG-adw、PLCTB-adw。
- PTEN and CB co expression was found in 8 cases, and there was difference in high and low differentiation group (differentiation group). PTEN和 CB共同表达者 8例 ;其中低分化组 1例 ;在高分化组中 7例 ;两者相比差异有显著性意义 (P<0 .;0 5 )
- AIM: To construct pET32a/His MBL-CLR recombinant prokaryotic expression plasmid and to express mannan-binding lectin-CLR (MBL-CLR) protein in E. 目的:在大肠杆菌中表达人甘露聚糖结合凝集素(MBL)胶原样区(CLR)蛋白。
- Methods: 1.The KK gene was directional cloned into the pAAV-MCS. Thisrecombinant pAAV expression plasmid was called pAAV-KK. 方法:1.;将KK 基因定向克隆入腺相关病毒载体质粒pAAV-MCS 中构建成pAAV-KK。
- Objective To investigate the correlation of the co expression of P glycoprotein (P gp) a multidrug resistance gene product and P53 protein in gastroenteric adenocarcinomas. 目的 探讨多药耐药基因产物P糖蛋白 (P glycoprotein,P gp)和P5 3蛋白在胃肠道腺癌中协同表达的意义。
- To construct mammal expression plasmid pcDNA 3.1 ( + )/GDF-5 and check the expression of it in bone marrow mesenchyal stem cells of mice. 目的:通过基因重组技术体外构建真核表达质粒pcDNA3.;1(+)/GDF-5;并检测其在小鼠骨髓基质干细胞中的表达。
- Conclusion:There are high rate of tumor cell proliferation in the co expression of C erbB 2,EGFR and P21 protein and the co expression of C erbB 2 and EGFR. 结果 :3种癌基因蛋白全部阳性及C erbB 2和EGFR同时阳性的胆囊癌细胞PCNA指数高于 3种蛋白全部阴性组 (P <0 .;0 5 )。 结论 :C erbB 2、EGFR和P2 1蛋白共同表达及C erbB 2和EGFR共同表达可能对胆囊癌细胞增殖有协同作用
- Methods:The epf gene fragment was amplified by PCR from ZYH24 and cloned into prokaryotic expression plasmid pGEX4T-2 to form pGEX4T-2-epf. 方法根据GenBank S.;suis2epf基因序列设计引物;克隆ZYH24株epf基因片段并进行序列分析;
- Then the BLY cDNA fragment was subcloned into prokaryotic expression vector pGEX-4T-1, forming the prokaryotic expression plasmid (pG-BLY). 克隆PCR产物，并构建了pGEX-4T-1-BLY原核表达载体。 经BamHI和EcoRI酶切及质粒PCR鉴定，证实本实验构建的新型牛溶菌酶基因已克隆到原核表达载体pGEX-4T-1上，为进一步研究其诱导表达条件及生物学功能奠定了基础。
