Cells after twice adhesions to culture flask were collected and labeled with CD14 and CD4.A is isotype control, B shows CD14 expression ratio (85.7%).

• 通过流式细胞仪检测分析单核细胞CD14标记显示，本研究中单核细胞的分离富集效率为76%25-88%25，图1为1次单核细胞分离后流式细胞仪检测单核细胞标记CD14和CD4的表达情况。