CLSM system can accurately reflect cytosolic concentration of free Ca 2+ labeled by Fluo-3/AM and Pluronic F-127. TCDD may increase [Ca 2+]_ i, then make some impairment to the heart function of the neonate rats.

• 应用Fluo3/AM和PluronicF127结合CLSM技术可以精确的反映细胞内游离钙离子浓度,TCDD可能通过某种机制增加心肌细胞[Ca2+]i,从而对乳鼠心脏功能产生一定损伤。