By using Mg/EGTA method, to centrifuge with different speeds, the BBMV in midgut of H. armigera could be successfully separated, and most of the APN activities in BBMV were preserved.

• 利用Mg/EGTA方法，以不同的速度进行离心，可以成功地分离棉铃虫中肠BBMV，且BBMV中保留了大部分APN活性。