By the cleaving with Xmal/SacI, the pac-1 gene was lignated with the vector pBI121, which was used in the transformation of plant by transfection of Agrobaterium. The plant expression vector pBI-121-pac-#1 was also constructed.

• 通过Xma I/Sac I双酶切将pac-1基因插入用于农杆菌转化的植物表达载体pBI121，构建了植物表达载体pBI-121-pac-1~(%232)。