By the PCR method,we got a fragment about 1300bp,sequencing and homology analysis result showed that the sequence was 1307 bp. Comparison the CHS cDNA and DNA sequence showed that the DNA have two extons interrupted by a 82bp intron.

• 获得了百合查尔酮合成酶基因(CHS)的DNA全长序列在获得CHS cDNA序列的基础上,重新设计CHS基因的引物,以基因组DNA为模板进行PCR扩增,获得了大约1300bp的目的片段,经克隆测序后表明,该片段长1307bp,与已得到的cDNA序列对比分析,CHS基因DNA序列包含两个外显子和1个内含子。