By means of LSCM based on H_2DCF-DA and DAF-2 DA, the specific molecular probes of H_2O_2 and NO respectively, the work provide evidence that in darkness the endogenous H_2O_2 fluorescence is very striking as well as NO fluorescence over the light.
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- 实验用H_2O_2和NO特异性荧光探针结合LSCM得到结果表明,暗中内源H_2O_2和NO的荧光很显著,而光下较低。