By designing four primers for an overlapping PCR, we created a fusion gene Ddl cat encoding human TNF receptor I death domain and chloramphenicol acetyltransferase(CAT).

• 通过设计4个引物进行重叠（overlapping）PCR，由此克隆了人肿瘤坏死因子受体I死亡域（deathdomain）与氯霉素乙酰转移酶（CAT）的融合蛋白基因（DdLcat）。