But the random ascospore progeny may be contaminated by conidia while conducting single ascus and ascospore isolation, which may result in a genetic bias.

• 采用先分离单个子囊，待其长成菌落并产生分生孢子后，再分离单个萌发的分生孢子的方法构建稻瘟病菌有性后代随机群体。