Blood samples were collected at 0, 6, 12, 24, 48, 72, 96 and 120hours after immunization, FSH, LH, E2 and P levels in serum were determined using RIA.

• 测定注射后0、6、12、24、48、72、96、120小时血液中FSH、促黄体素释放激素（LH）、雌二醇(E2)和孕酮(P)的含量。