Based on the preparation of sensitive, specific DIG labeled probe through random primed labeling, a protocol of in situ RT-nested-PCR-ISH for the detection of H5N1 AIV was established.

• 在利用随机引物标记法制备特异DIG 标记探针的基础上，建立了一套检 测H5N1 亚型禽流感病毒的in situ RT-nested-PCR-ISH 方法。