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- Keywords barley yellow dwarf virus （BYDV） GPV,micro projectile bombardment,pollen tube pathway,wheat transformation,tissue culture; 大麦黄矮病毒;基因枪;花粉管通道;小麦转化;组织培养;
- BYDV GPV 大麦黄矮病毒GPV株系
- These fragments are related to resistance to BYDV. 可以认为这些谱带与大麦黄矮病抗性有关。
- The coat protein gene of GPV, a Chinese serotype isolate of BYDV, was used to construct three plant expression vectors, pPPI 2, pPPI 3 and pPPI 5, which contain Act promoter or Emu promoter respectively. 以我国特有的大麦黄矮病毒 GPV株系的外壳蛋白 ( CP)基因为材料 ,设计合成了分别含有 Act启动子或 Emu启动子的植物表达载体 p PPI2、p PPI3和 p PPI5。
- GPV and ACP couldn't catch the last flight today, due to delay of delivery. 今天送GPV及ACP的货由于出货延迟;已赶不上今天最后一班航班.
- The genetic behavior of an agropyron intermedium chromosome conferring BYDV resistance in wheat background. 小麦背景下BYDV抗性携带染色体的遗传行为。
- T1 plants of the transgenic lines (2 and 3) were indicated resistance to GPV to some extent. 获得的2个转基因系的T1代苗在温室中的初步抗病检测显示，转基因后代对GPV株系具有一定抗性。
- This paper discusses the importance of BYDV resistance from scientific viewpoint. 本文论证了全球小麦抗黄矮病育种的重要性；
- According to nucleotide suquence of GPV A strain,a pairs of primers were designed and synthesized,and mutated the VP2ACG codon to ATG one. 根据国外已发表的鹅细小病毒(GPV)A株基因组核苷酸序列,设计并合成了一对用于GPV主要结构蛋白(VP2-VP3)基因表达的引物。
- According to the published sequence of GPV B strain in GenBank, a pair of primers was designed by Oligo4.0. NS1 gene of GPV was amplified by PCR. 本研究根据 Gen Bank发表的 GPV B株全基因核苷酸序列 ,设计了 1对用以扩增 GPV主要非结构蛋白 NS1基因的引物。
- The seedlings infected by BYDV, win suffer a light degree dwarfing, and the resistance of maize varieties to BYDV are different in the seedling stage. BYDV侵染玉米可造成轻度矮化，且不同玉米品种苗期对BYDV的抗性也存在差异。
- The gene shared 98.50% and (93.18%) homology in (bases), and shared 98.09% and 95.77% respectively in amino acid with GPV strains B and YG. 经与B株、YG株进行同源性比较 ;核苷酸的同源性分别为 98.;5 0%25和 93
- The chromosome conferring resistance to BYDV showed the intendance to substitute 2D rather than 2A, 2B wheat chromosomes in disomic substutution lines. 在二体代换系中,该染色体优先取代2D小麦染色体、而非2A、2B小麦染色体;
- The high conservative main antigenic protein VP3 of GPV provided the access to producing the new type vaccines and diagnostic agent. GPV主要免疫原性蛋白VP3的高度保守性给新型疫苗及分子诊断试剂的研制提供了思路。
- Variance coefficients of BYDV resistance value reduces rapidly when generation added, and BYDV resistance gene purify rapidly. 抗性变异随世代的增加减幅较大，抗病基因纯合较快；
- The Seedling of maize Varieties were inoculated with mix toxogen of BYDV or SGV、RPV、MAV (three strains of BYDV) in the green house and the experimental field. 用大麦黄矮病毒(BYDV)的混合毒原及分离的4种株系，分别在温室和田间接种不同玉米品种幼苗。
- But the comparison amino acid sequence shows that GPV DNA has high homology with the human adeno-associated virus AAV-2 in dependovirus and B19 in erythrovirus. GPV与依赖病毒属成员AAV-2及红病毒属成员人细小病毒B19的进化关系较近。
- The wheat new line Longfu97K1099 with resistance to BYDV was selected by means of irradiation and introduction exogenous DNA via the pollen-tube pathway. 利用辐射与外源DNA花粉管导入技术相结合的方法，选出了抗大麦黄矮病(BYDV)的小麦新品系龙辐97K1099。
- These MAbs only reacted with GPV, but not with other sepecies like CPV, FPV and PPV in Parvovirus, and other unrelated avian viruses such as NDV, IBDV, DHV and DPV in indirect ELISA. 这些单抗仅与GPV反应，与其它细小病毒（CPV、FPV、PPV）和其它禽病毒（NDV、IBDV、DHV、DPV）均不反应。
- A pair of primers was designed to amplify VP1 gene of GPV by PCR according to the published sequence of GPV B strain in GenBank. The product of PCR whose length is 2.2 kb was cloned to the pMD 18-T vector,then made sequencing and analyzing . 参照GenBank中收录的鹅细小病毒 (GPV)B株基因序列设计并合成了扩增GPVH1株VP1的 1对引物 ;利用PCR技术扩增出长约 2 .;2kb的目的片段;将其克隆到 pMD 18 T载体上;进行了序列测定及分析。
