At the same time, atrB encoding phosphotransferase and atrC encoding 4-aminobutyrate aminotransferase were cloned, and atrA, artB and artC were clustered in a cluster. atrB and atrC mutants were constructed by insertion of km cassettes, respectively.

 
  • 另外还克隆了atrA下游的atrB和atrC基因,它们分别编码磷酸转移酶和氨基转移酶,atrA、artB和artC共同组成了一个基因簇,推测此基因簇参与催化吲哚丙酮酸途径中由色氨酸脱氨基生成吲哚丙酮酸的酶促反应。
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