At the end of 4-week test, chromosome Y in the female mice was determined by PCR and Cy3 labeled insulin and CFSE staining were observed under fluorenscent microscope.

• 观察各组小鼠血糖、体重变化。 4周后实验结束时,取胰腺行Y染色体PCR扩增、胰岛素免疫荧光和CFSE检测,了解骨髓干细胞在胰腺的定位及诱导分化情况;