As a result, the stable, simple, fast method of two-temperature gradient PCR for sexing bovine pre-implantation embryos was obtained, which only took 57 minutes to identify the embryo sex.

• 通过两温度梯度PCR方法对牛基因组、克隆胚胎、胚胎样品进行性别鉴别实验研究，建立了稳定、简便、快速的牛早期胚胎性别鉴别两温度梯度PCR方法，鉴定时间仅为57分钟。