As a result of this mechanism, the proteins are separated in an unorthodox way, with the smaller proteins/peptides staying closer to the application point and larger proteins migrating further down toward the anodic gel end.

• 因为此一分离机理，在分离蛋白质时其方式也不同与传统方法，小分子蛋白质或肽段保留在点样点附近而大分子蛋白质向阳极端迁移距离较长。