Apoptosis of keratinocytes was determined by the terminal deoxynucleotidyl transferase end-labeling(TUNEL)assay. The expression of Bax,Bcl-2 and Caspase 3 was evaluated by immunohistochemistry.

• 采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记技术(TUNEL)检测角质形成细胞的凋亡状况,同时采用免疫组化法检测角质形成细胞表达凋亡相关蛋白Bax、Bcl-2和Caspase 3的情况。