And then,the arteries were kept in bicarbonate buffer [ebs-Henseleit(KH,control group)],high potassium solution(20, 50 mmol/L,group A or B) or KH solution plus NCR(0.1 mmol/L),high potassium solution(20, 50 mmol/L,group C,D or E)for 4 hours.

• 在4℃条件下分别用重碳酸盐缓冲液(KH液；对照组)、高钾(20；50mmol/L；A；B组)溶液、添加尼可地尔(0.；1mmol/L)的KH液、高钾(20；50mmol/L)溶液(C；D；E组)保存4h。