And the competitive ELISA was found to detect HMGB1 ranging from 1 to 320 ng/ml and to have a recovery ranging form 97% to 106% and a CV from 2.6% to 7.3%.

• 建立的直接竞争ELISA方法的测定范围为1~320 ng/ml；回收率为97%25~106%25；变异系数为2.；6%25~7