And cloning to the expression vector pET-32a(+) fused with Trx-Tag coding sequence or not, to acquire the recombinant plasmid trx-fnb- pET-32a(+), trx-cna- pET-32a(+),fnb- pET-32a(+) and cna- pET-32a(+).

 
  • 并克隆于原核表达载体pET-32a(+),分别构建了融合基因表达质粒trx-fnb-pET-32a(+),trx-cna-pET-32a(+)和非融合基因表达质粒fnb-pET-32a(+),cna-pET-32a(+);
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