An infrared/attenuated total reflection (ATR) technique has been utilized to study the conformational changes of lipase induced by nonaqueous solvents, without the need of dissolving the protein in the nonaqueous solvent.

• 利用衰减全反射（AtenuatedTotalReflection，ATR）傅立叶变换红外光谱研究了脂肪酶在不同非水有机溶剂中的构象变化。