An indirect enzymelinked immunosorbent assay (ELISA) was developed to detect the antibody valence of DRV. The antigen was prepared for ELISA by sodium sulfate centrifugation and precipitation and its virus protein content was up to 0.7355 mg /ml.

• 建立间接ELISA方法以检测禽(番鸭)呼肠孤病毒抗体滴度。 经二次差速离心、盐析法浓缩提纯细胞毒，获得浓度为0.；7355mg/ml病毒抗原液。