An asymmetric PCR profile was employed with setting of one high and one low annealing temperature for RAMP-PCR during the last 15 cycles, and REMAP-PCR program was as usual.

• 而RAMP分析采用不对称PCR循环，在PCR扩增的后15个循环中，退火温度设为2个；REMAP则采用常规PCR循环。