Among the above three cryoprotectants, trehalose showed the best antifreezing effect, while polydextrose and sodium lactate showed almost the same effect as commercial cryoprotectants.

• 三种抗冻剂中，以海藻糖的抗冻效果最好，聚葡萄糖和乳酸钠的效果与商业抗冻剂相似。