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- Conclusion The SNP fluorescent-multiplex system based on the fragment length discrepant allele specific PCR strategy is simple and economical, and is of a high application value in forensic medicine. 结论采用荧光标记复合扩增毛细管电泳法进行SNP分型，方法简单实用，在法医学个人识别领域具有较高的应用。
- Methods:Polymerase chain reaction in combination with dot blot hybridization of allele specific oligonucleotide(PCR/ASO) probes was used. 方法：采用聚合酶链反应结合等位基因特异的寡核苷酸探针杂交（PCR/ASO）技术。
- We genotyped some main Chinese sweet cherry cultivars for the first time using the alleles specific PCR, and two new S genotype (S1S6, S4S9) were found, which made the listing of S-allele assignment more completed. 对57个国内外的甜樱桃品种进行了自交不亲和(SI)基因型的鉴定，首次鉴定了我国主栽的一些甜樱桃品种的S基因型，并发现了两个新的S基因型S1S6、S4S9，将自交不亲和的组群扩展到21个。
- Keywords Plasmodium vivax;circumsporozoite protein（CSP）;nest allele specific PCR;different size DNA band; 间日疟原虫;环子孢子蛋白基因;套式等位特异PCR;多样性DNA片段;
- Keywords Forensic biological evidence;Y chromosome;Biallelic marker;Tragment length discrepant allele specific PCR;Haplogroup; 法医物证学;Y染色体;双等位基因标记;片段长度差异等位基因特异性PCR;单体群;
- allele specific amplification(ASA) 等位基因特异性扩增
- allele specific oligonucleotide(ASO) 等位基因特异性寡核苷酸, 等位基因特异的
- allele specific oligonucleotide hybridization 等位基因特异寡核苷酸杂交
- Allele specific polymerase chain reaction 等位基因特异性聚合酶链反应
- Keywords allogeneic hematopoietic stem cell transplantation;chimerism;single nucleotide polymorphism;real-time PCR;phosphorothioate modified;allele specific amplification; 异基因造血干细胞移植;嵌合体;单核苷酸多态性;实时荧光PCR;硫代化修饰;等位基因特异性扩增;
- Single-tube bi-directional allele specific amplification 单管双向等位基因专一性扩增
- The mutation was confirmed by allele speci fic PCR (ASPCR). 应用等位基因特异性PCR验证测序所发现的突变。
- Education should not be restricted to any one specific age group. 教育不应限制在任何特定的年龄组上。
- allele specific PCR 等位基因特异性多聚酶链反应
- allele specific PCR(AS-PCR) 等位特异性PCR, 等位基因特异性扩增技术
- Allele specific - PCR 等位基因特异性-PCR
- He gave us very specific instructions. 他给我们做了非常明确的指示。
- There is a specific tool for each job. 各工种配备特定的工具。
- But that all depend on the specific circumstances. 不过，那要看具体情况而定。
- He held fire on specific foreign policy question. 他对具体的外交政策暂不表态。
