After digestion by EcoRI and SalI, the PCR products were inserted to pUC20 vector for sequence analysis, which showed that the amplified VP72 gene was identical to that of ASF BA71 strain.

• 将测序正确的VP72和VP72S基因克隆入pGEX-6p-1载体中GST基因的下游,重组大肠杆菌用IPTG诱导表达,并采用不同方法纯化得GST融合蛋白。