After anesthesia, histamine and saline were injected into local back, and then bladde reaction (Evans blue leakage) appeared around cutaneous stimulation area.

• 鼠皮肤炎症模型的建立:从BALB/c鼠尾静脉注射标定浓度的Evans蓝,麻醉后在脊背局部皮内分别注射组胺及生理盐水,随后在皮肤刺激区出现水泡反应(Evans蓝渗漏)。