After IPTG induction, there was a new protein band about Mr 24kD on SDS-PAGE. The percent expressed product over total bacterial proteins after 1, 2, 3, and 4 hours of induction was 18.2%, 18.8%, 23.0% and 23.4%, respectively, estimated by densitometry.

• IPTG诱导蛋白表达；SDSPAGE电泳分析表明在相对分子质量24kD左右出现新的蛋白表达条带；诱导后1h、2h、3h、4h表达蛋白占菌体总蛋白的百分比分别为18.；2%25、18